Multi-modality imaging of structure and function combining spectral-domain optical coherence and multiphoton microscopy

Claudio Vinegoni; Tyler Ralston; Wei Tan; Wei Luo; Daniel L. Marks; Stephen A. Boppart

doi:10.1117/12.648745

20 February 2006 Multi-modality imaging of structure and function combining spectral-domain optical coherence and multiphoton microscopy

Claudio Vinegoni, Tyler Ralston, Wei Tan, Wei Luo, Daniel L. Marks, Stephen A. Boppart

Author Affiliations +

Proceedings Volume 6079, Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine X; 60791D (2006) https://doi.org/10.1117/12.648745
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

We have constructed an integrated optical microscope that allows for the simultaneous image acquisition from multiple optical imaging modalities. The microscope consists primarily of hardware for spectral-domain optical coherence microscopy (OCM), multi-photon microscopy (MPM), and second harmonic generation microscopy. The unique configuration of the integrated microscope allows for the acquisition of both anatomical and functional imaging information with particular emphasis in the fields of tissue engineering and tumor biology. By overlaying the structural image obtained from OCM on the functional image obtained simultaneously from MPM (i.e., fluorescent markers imply functional proteins), a more comprehensive view of different tissues can be obtained. In addition, the contemporary analysis of the spectroscopic features enhances contrast in OCM by differentiating different cell and tissue components.

Citation Download Citation

Claudio Vinegoni, Tyler Ralston, Wei Tan, Wei Luo, Daniel L. Marks, and Stephen A. Boppart "Multi-modality imaging of structure and function combining spectral-domain optical coherence and multiphoton microscopy", Proc. SPIE 6079, Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine X, 60791D (20 February 2006); https://doi.org/10.1117/12.648745

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