Application of PIV technique in suspension biochip detection

Dengke Zhang; Xuxiang Ni; Yan Shi

doi:10.1117/12.710732

27 October 2006 Application of PIV technique in suspension biochip detection

Dengke Zhang, Xuxiang Ni, Yan Shi

Proceedings Volume 6047, Fourth International Conference on Photonics and Imaging in Biology and Medicine; 60473O (2006) https://doi.org/10.1117/12.710732
Event: Fourth International Conference on Photonics and Imaging in Biology and Medicine, 2005, Tianjin, China

Abstract

Movement in fluid field is a significant facet in suspension biochip detection and can be analyzed by the non-intrusive technique-Particle image velocimetry (Ply). A special flat channel oftest sample was designed in the suspension biochip detecting system to form a two-dimension fluid field. Serial images ofthe suspension biochip fluid field were acquired by high-sensitivity CCD at ten frames per second and analyzed through Ply technique based on cross-correlation. First, image was preprocessed to remove noise and became a binary image. Second, the preprocessed image was divided into a proper number of area units, and then velocity vector was obtained by calculating the cross-correlation of corresponding areas between two images. All velocity vectors were synthesized to reconstruct a whole velocity distribution map of fluid field. Two simulated particle images have been made and the velocity distribution was reconstructed based on PIV technique. The result shows that PIV technique can effectively obtain velocity distribution offluid field through which we can improve the structure of fluid field channel and accomplish continuous analysis by processing different area in two-dimension fluid field according to their relative velocity.

Citation Download Citation

Dengke Zhang, Xuxiang Ni, and Yan Shi "Application of PIV technique in suspension biochip detection", Proc. SPIE 6047, Fourth International Conference on Photonics and Imaging in Biology and Medicine, 60473O (27 October 2006); https://doi.org/10.1117/12.710732

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