Beyond monochromatic light: three-dimensional confocal laser scanning microscopy using a supercontinuum source

G. McConnell; J. M. Girkin; S. Poland

doi:10.1117/12.710991

27 October 2006 Beyond monochromatic light: three-dimensional confocal laser scanning microscopy using a supercontinuum source

G. McConnell, J. M. Girkin, S. Poland

Author Affiliations +

Proceedings Volume 6047, Fourth International Conference on Photonics and Imaging in Biology and Medicine; 60472Q (2006) https://doi.org/10.1117/12.710991
Event: Fourth International Conference on Photonics and Imaging in Biology and Medicine, 2005, Tianjin, China

Abstract

Confocal laser scanning microscopy (CLSM) has rapidly become an essential tool in the life sciences laboratory, enabling high-resolution and minimally intrusive optical sectioning of fluorescent samples. Most commercially available CLSM systems employ a gas laser, e.g. a Kr/Ar laser, to provide the excitation radiation. However, such lasers have several shortcomings, including the maintenance requirements, short lifetimes and high noise levels. To overcome these limitations, a light source for CLSM that is based on supercontinuum generation in photonic crystal fiber has been developed. This source provides the necessary wavelength range required to excite the widest possible variety of fluorophores. A novel method of extracting the desired wavelengths from the supercontinuum source using a digital micro-mirror device (DMD) is also described.

Citation Download Citation

G. McConnell, J. M. Girkin, and S. Poland "Beyond monochromatic light: three-dimensional confocal laser scanning microscopy using a supercontinuum source", Proc. SPIE 6047, Fourth International Conference on Photonics and Imaging in Biology and Medicine, 60472Q (27 October 2006); https://doi.org/10.1117/12.710991

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