Fluorescence lifetime imaging microscopy using a tunable continuum source and a Nipkow disk confocal microscope

D. Grant; E. Auksorius; D. Schimpf; D. S. Elson; C. Dunsby; J. Requejo-Isidro; I. Munro; M. A. A. Neil; P. M. W. French; P. Courtney

doi:10.1117/12.632952

7 October 2005 Fluorescence lifetime imaging microscopy using a tunable continuum source and a Nipkow disk confocal microscope

D. Grant, E. Auksorius, D. Schimpf, D. S. Elson, C. Dunsby, J. Requejo-Isidro, I. Munro, M. A. A. Neil, P. M. W. French, P. Courtney

Author Affiliations +

Proceedings Volume 5860, Confocal, Multiphoton, and Nonlinear Microscopic Imaging II; 58600K (2005) https://doi.org/10.1117/12.632952
Event: European Conferences on Biomedical Optics 2005, 2005, Munich, Germany

Abstract

Multi-beam confocal sectioned fluorescence lifetime imaging microscopy is demonstrated using a Yokogawa spinning disk. The single-photon excitation source is a supercontinuum generated from a Ti:sapphire seeded photonic crystalline fibre.

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D. Grant, E. Auksorius, D. Schimpf, D. S. Elson, C. Dunsby, J. Requejo-Isidro, I. Munro, M. A. A. Neil, P. M. W. French, and P. Courtney "Fluorescence lifetime imaging microscopy using a tunable continuum source and a Nipkow disk confocal microscope", Proc. SPIE 5860, Confocal, Multiphoton, and Nonlinear Microscopic Imaging II, 58600K (7 October 2005); https://doi.org/10.1117/12.632952

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