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Autofluorescence-endogenous fluorescence of cells and tissues has been known for many years. For a long time it has been considered mostly a phenomenon producing unwanted background impairing a quality of fluorescence microscopic images. First reports on a diagnostic potential of the autofluorescence for a detection of premalignant and malignant lesions were published only at the end of 1990. This paper presents basic ideas of such techniques and their applications in the field of tumor detection.
B. W. Chwirot andS. Chwirot
"Endogenous fluorescence: new approach to in situ detection of premalignant and malignant lesions in human organs", Proc. SPIE 5849, Fifth Workshop on Atomic and Molecular Physics, (1 June 2005); https://doi.org/10.1117/12.629462
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B. W. Chwirot, S. Chwirot, "Endogenous fluorescence: new approach to in situ detection of premalignant and malignant lesions in human organs," Proc. SPIE 5849, Fifth Workshop on Atomic and Molecular Physics, (1 June 2005); https://doi.org/10.1117/12.629462