Dark fraction and blinking of water-soluble quantum dots in solution

Jie Yao; Daniel R. Larson; Warren R. Zipfel; Watt W. Webb

doi:10.1117/12.606131

28 April 2005 Dark fraction and blinking of water-soluble quantum dots in solution

Jie Yao, Daniel R. Larson, Warren R. Zipfel, Watt W. Webb

Proceedings Volume 5705, Nanobiophotonics and Biomedical Applications II; (2005) https://doi.org/10.1117/12.606131
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

Water-soluble quantum dots (qdots) have been introduced as bright fluorophores into life sciences research. Although various photophysical pathologies of qdots have been found, how their biological applications will be affected --- particularly in the native biological environment --- has not been evaluated. By fluorescence coincidence analysis and fluorescence cross-correlation spectroscopy, we studied the dark fraction of free-diffusing qdots in aqueous solution. We were able to detect individual qdots and found significant heterogeneity --- well-distinguished dark qdots and bright qdots. We estimated the bright fraction of Qdot525-Streptavidin to be about 55%. Blinking events were also noticed in fluorescence coincidence analysis, with “on/off” timescale from submilliseconds to tens of milliseconds.

Citation Download Citation

Jie Yao, Daniel R. Larson, Warren R. Zipfel, and Watt W. Webb "Dark fraction and blinking of water-soluble quantum dots in solution", Proc. SPIE 5705, Nanobiophotonics and Biomedical Applications II, (28 April 2005); https://doi.org/10.1117/12.606131

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available