Metal nanoparticles as passive and active tools for bioanalytics

Wolfgang Fritzsche; Andrea Csaki; Andrea Steinbrueck; Frank Garwe; Karsten Koenig; Markus Raschke

doi:10.1117/12.590501

29 March 2005 Metal nanoparticles as passive and active tools for bioanalytics

Wolfgang Fritzsche, Andrea Csaki, Andrea Steinbrueck, Frank Garwe, Karsten Koenig, Markus Raschke

Proceedings Volume 5699, Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III; (2005) https://doi.org/10.1117/12.590501
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

Metal nanoparticles represent an interesting tool for bioanalytics. Due to their small size, attachment to biomolecules does not interfere significantly with specific molecular binding. Therefore particles can be applied as label in affinity assays (e.g., DNA hybridization), using setups with high parallelization. Beside this rather passive use of nanoparticles, these structures can also be utilized as 'nano antenna' for the conversion of laser light pulses into heat. Using DNA-modified particles sequence-specific bound to DNA, a novel restriction technique is in development that applies this conversion for local DNA destruction. Metal nanoparticles combine the ability for highly precise positioning (due to specific molecular binding) with the possibility of optical access in a bright-field mode. They exhibit an interesting potential for spanning the gap between the macroscopic technical environment and the molecular scale, thereby enabling a true integration of nanoscale constructs with today’s technology.

Citation Download Citation

Wolfgang Fritzsche, Andrea Csaki, Andrea Steinbrueck, Frank Garwe, Karsten Koenig, and Markus Raschke "Metal nanoparticles as passive and active tools for bioanalytics", Proc. SPIE 5699, Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III, (29 March 2005); https://doi.org/10.1117/12.590501

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