Automated high-throughput micro-injection system for floating cells

Satoru Sakai; Sachihiro Youoku; Yoshinori Suto; Moritoshi Ando; Akio Ito

doi:10.1117/12.590014

29 March 2005 Automated high-throughput micro-injection system for floating cells

Satoru Sakai, Sachihiro Youoku, Yoshinori Suto, Moritoshi Ando, Akio Ito

Proceedings Volume 5699, Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III; (2005) https://doi.org/10.1117/12.590014
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

We have developed an automated microinjection system that captures many floating cells and controls capillary positions precisely. To capture many cells simultaneously, we constructed an array of holes on a 10 x 10 mm silicon-based substrate. The hole diameter is 3 μm because our target cells are 10 - 20 μm in diameter. A suction pump connected to the bottom of the multi-hole silicon chip draws the medium into the holes using a slight vacuum, so cells are caught there. Using an initial prototype chip having 121 holes, we captured over 90 cells in a single sweep. Automated microinjection requires precise control of the capillary positions, so images of the capillary and holes on the chip are observed using a microscope with a CCD camera located above the biological medium. The 3D positions of these elements are accurately measured by processing these images. The capillary and the chip are mounted on automatic stages individually controlled with this position data. Using these techniques, this system can microinject about one cell per second. Its success rate for microinjection is 61% for PC12 cells.

Citation Download Citation

Satoru Sakai, Sachihiro Youoku, Yoshinori Suto, Moritoshi Ando, and Akio Ito "Automated high-throughput micro-injection system for floating cells", Proc. SPIE 5699, Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III, (29 March 2005); https://doi.org/10.1117/12.590014

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