Study pollen grains in rice by using multispectral imaging techniques

Qiongshui Wu; Yaojun Hu; Hengyu Ke; Libo Zeng; Yi Ding

doi:10.1117/12.589589

23 March 2005 Study pollen grains in rice by using multispectral imaging techniques

Qiongshui Wu, Yaojun Hu, Hengyu Ke, Libo Zeng, Yi Ding

Proceedings Volume 5694, Spectral Imaging: Instrumentation, Applications, and Analysis III; (2005) https://doi.org/10.1117/12.589589
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

This paper describes a novel multispectral imaging microscope and its applications in the study of pollen grains in rice. The Imaging instruments can simultaneously record both spectral and spatial information of a sample, which is helpful to study the chemical states and physical properties of the sample by taking advantage of spatial image processing and spectroscopic analysis techniques. A LCTF (liquid crystal tunable filter) device is used for fast wavelength selection in the range of 400nm to 720nm and a cooled two-dimensional monochrome CCD for image detection. In this paper, the image acquisition process, spatial and spectral calibration and spectral imaging analysis methods are detailed. And also a novel method using this multispectral imaging microscope to observe rice pollen grains is reported here. The multispectral images were systematically processed and analyzed by the software. The results illustrated that the transmittance analysis of multispectral pollen images can accurately identify the pollen abortion stage of male-sterile rice, and can easily distinguish a variety of male sterile cytoplasm. Compared with cytological and histochemical methods reported previously, the method reported here has demonstrated to be more efficient and reliable in the study of chemical states and physical properties in plant cells.

Citation Download Citation

Qiongshui Wu, Yaojun Hu, Hengyu Ke, Libo Zeng, and Yi Ding "Study pollen grains in rice by using multispectral imaging techniques", Proc. SPIE 5694, Spectral Imaging: Instrumentation, Applications, and Analysis III, (23 March 2005); https://doi.org/10.1117/12.589589

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
10 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Multispectral imaging

Transmittance

Chemical analysis

Image processing

Microscopes

Image analysis

Image transmission

Show All Keywords

Keywords/Phrases

Search In:

Publication Years