Real-time imaging of membrane potentials during exposure to nanosecond pulsed electric fields

Juergen F. Kolb; Wolfgang Frey; Jody A. White; R. O. Price; Peter F. Blackmore; Stephen J. Beebe; Ravindra P. Joshi; Karl H. Schoenbach

doi:10.1117/12.591313

1 April 2005 Real-time imaging of membrane potentials during exposure to nanosecond pulsed electric fields

Juergen F. Kolb, Wolfgang Frey, Jody A. White, R. O. Price, Peter F. Blackmore, Stephen J. Beebe, Ravindra P. Joshi, Karl H. Schoenbach

Author Affiliations +

Proceedings Volume 5692, Advanced Biomedical and Clinical Diagnostic Systems III; (2005) https://doi.org/10.1117/12.591313
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

The charging of mammalian cell plasma membranes in response to ultrashort pulsed electric fields of 60 ns and field strengths up to 100 kV/cm was investigated. Membranes of Jurkat cells were stained with a potential-sensitive dye, Annine-6 and placed in a microreactor mounted on an inverted fluorescence microscope. Images of changes in the fluorescence intensity during the exposure were recorded with a high-sensitivity CCD-camera. A temporal resolution of 5 ns was achieved by illuminating the cells with a 5 ns laser pulse from a dye-laser. The laser pulse was synchronized with the high voltage pulse to record images at specific times before, during and after exposure to the electric field. When exposing Jurkat cells to a 60 ns, 100 kV/cm pulse, each hemisphere of the plasma membrane (as oriented with respect to the electrodes) responded uniquely to the applied field. From these observations it is possible to draw conclusions on the charging time of the membrane, maximum transmembrane voltages and the onset of poration.

Citation Download Citation

Juergen F. Kolb, Wolfgang Frey, Jody A. White, R. O. Price, Peter F. Blackmore, Stephen J. Beebe, Ravindra P. Joshi, and Karl H. Schoenbach "Real-time imaging of membrane potentials during exposure to nanosecond pulsed electric fields", Proc. SPIE 5692, Advanced Biomedical and Clinical Diagnostic Systems III, (1 April 2005); https://doi.org/10.1117/12.591313

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available