Fluorescence detection of proteins released by Bacillus subtilis spores during heat shock germination

Alexandra Alimova; Alvin Katz; Misu Paul; Elizabeth Rudolph; Paul Gottlieb; J. C. Steiner; Robert R. Alfano

doi:10.1117/12.588592

1 April 2005 Fluorescence detection of proteins released by Bacillus subtilis spores during heat shock germination

Alexandra Alimova, Alvin Katz, Misu Paul, Elizabeth Rudolph, Paul Gottlieb, J. C. Steiner, Robert R. Alfano

Proceedings Volume 5692, Advanced Biomedical and Clinical Diagnostic Systems III; (2005) https://doi.org/10.1117/12.588592
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

Fluorescence and absorption spectroscopy were performed on Bacillus subtilis spores which were heat treated at temperatures ranging from 20°C to 90°C. The tryptophan (trp) emission from the spore suspensions treated at temperatures greater than 60°C was shifted towards longer wavelength as compared to the spores which were heat treated at lower temperatures. These spectral changes were the result of proteins released by the spores into the suspension. Trp residues in the emitted proteins are in a more polar environment and therefore exhibit a larger Stokes shift. Fluorescence and absorption measurements show that the concentration of proteins in the supernatant was greater for spores treated at higher temperatures. Electrophoresis gel analysis showed the presence of a 47 KDa protein in the supernatant.

Citation Download Citation

Alexandra Alimova, Alvin Katz, Misu Paul, Elizabeth Rudolph, Paul Gottlieb, J. C. Steiner, and Robert R. Alfano "Fluorescence detection of proteins released by Bacillus subtilis spores during heat shock germination", Proc. SPIE 5692, Advanced Biomedical and Clinical Diagnostic Systems III, (1 April 2005); https://doi.org/10.1117/12.588592

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