Depth-resolved fluorescence of human ectocervical tissue

Yicong Wu; Peng Xi; Tak-Hong Cheung; So Fan Yim; Mei-Yung Yu; Jianan Y. Qu

doi:10.1117/12.593826

1 April 2005 Depth-resolved fluorescence of human ectocervical tissue

Yicong Wu, Peng Xi, Tak-Hong Cheung, So Fan Yim, Mei-Yung Yu, Jianan Y. Qu

Proceedings Volume 5692, Advanced Biomedical and Clinical Diagnostic Systems III; (2005) https://doi.org/10.1117/12.593826
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

The depth-resolved autofluorescence of normal and dysplastic human ectocervical tissue within 120um depth were investigated utilizing a portable confocal fluorescence spectroscopy with the excitations at 355nm and 457nm. From the topmost keratinizing layer of all ectocervical tissue samples, strong keratin fluorescence with the spectral characteristics similar to collagen was observed, which created serious interference in seeking the correlation between tissue fluorescence and tissue pathology. While from the underlying non-keratinizing epithelial layer, the measured NADH fluorescence induced by 355nm excitation and FAD fluorescence induced by 457nm excitation were strongly correlated to the tissue pathology. The ratios between NADH over FAD fluorescence increased statistically in the CIN epithelial relative to the normal and HPV epithelia, which indicated increased metabolic activity in precancerous tissue. This study demonstrates that the depth-resolved fluorescence spectroscopy can reveal fine structural information on epithelial tissue and potentially provide more accurate diagnostic information for determining tissue pathology.

Citation Download Citation

Yicong Wu, Peng Xi, Tak-Hong Cheung, So Fan Yim, Mei-Yung Yu, and Jianan Y. Qu "Depth-resolved fluorescence of human ectocervical tissue", Proc. SPIE 5692, Advanced Biomedical and Clinical Diagnostic Systems III, (1 April 2005); https://doi.org/10.1117/12.593826

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
6 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Luminescence

Tissues

Tissue optics

Collagen

Pathology

Confocal microscopy

Fluorescence spectroscopy

Show All Keywords

Keywords/Phrases

Search In:

Publication Years