Femtosecond laser ablation of porcine intestinal mucosa: potential autologous transplant for segmental cystectomy

Russell G. Higbee; Bryan S. Irwin; Michael N. Nguyen; Yuanyuan Zhang M.D.; William L. Warren

doi:10.1117/12.591262

25 April 2005 Femtosecond laser ablation of porcine intestinal mucosa: potential autologous transplant for segmental cystectomy

Russell G. Higbee, Bryan S. Irwin, Michael N. Nguyen, Yuanyuan Zhang M.D., William L. Warren

Author Affiliations +

Proceedings Volume 5686, Photonic Therapeutics and Diagnostics; (2005) https://doi.org/10.1117/12.591262
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

Nearly 80% of patients with newly diagnosed bladder cancer present with superficial bladder tumors (confined to the bladder lining such as transitional cell carcinoma [90%], squamous cell carcinoma [6-8%], and adenocarcinoma[2%]) in stages Ta, Tis, or T1. Segmental cystectomy is one surgical treatment for patients who have a low-grade invasive tumor. Transposition of small intestine is a viable surgical treatment option. Success of the transplantation is also dependent upon removal of the entire SI mucosal layer. A Clark Spitfire Ti:Sapphire laser operating at 775 nm and 1 kHz repetition rate, was used to investigate the damage induced to fresh cadaveric porcine small intestinal mucosal epithelium. The laser was held constant at a focal spot diameter of 100 μm using a 200 mm focal point lens, with a power output maximum of 257 mW. A high resolution motorized X-Y-Z stage translated the SI tissue through the beam at 500 μm/sec with a line spacing of 50 μm. This produced a 50% overlap in the laser etching for each pass over a 1 cm x 1.5 cm grid. To determine if the mucosal lining of the SI was adequately removed, the targeted area was covered with 1% fluorescein solution for 30 seconds and then rinsed with phosphate buffered saline. Fluorescein staining was examined under UV illumination, to determine the initial degree of mucosal removal. Tissues were fixed and processed for light and scanning electron microscopy by standard protocols. Brightfield light microscopy of hematoxylin and eosin stained 4 μm thick cross sections, scanning electron microscopy were examined to determine the degree of mucosal tissue removal. Clear delineation of the submucosal layer by fluorescein staining was also observed. The Ti:Sapphire laser demonstrated precise, efficient removal of the mucosal epithelium with minimal submucosal damage.

Citation Download Citation

Russell G. Higbee, Bryan S. Irwin, Michael N. Nguyen, Yuanyuan Zhang M.D., and William L. Warren "Femtosecond laser ablation of porcine intestinal mucosa: potential autologous transplant for segmental cystectomy", Proc. SPIE 5686, Photonic Therapeutics and Diagnostics, (25 April 2005); https://doi.org/10.1117/12.591262

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