Adaptive SERS substrates for protein sensing

Mark D. Thoreson; Vladimir P. Drachev; Eldar N. Khaliullin; Vishal Nashine; Dor Ben-Amotz; V. Jo Davisson; Vladimir M. Shalaev

doi:10.1117/12.579779

29 December 2004 Adaptive SERS substrates for protein sensing

Mark D. Thoreson, Vladimir P. Drachev, Eldar N. Khaliullin, Vishal Nashine, Dor Ben-Amotz, V. Jo Davisson, Vladimir M. Shalaev

Author Affiliations +

Proceedings Volume 5593, Nanosensing: Materials and Devices; (2004) https://doi.org/10.1117/12.579779
Event: Optics East, 2004, Philadelphia, Pennsylvania, United States

Abstract

Adaptive surface-enhanced Raman scattering (SERS) substrates exhibit unique properties which make them well suited for SERS studies of proteins on surfaces. Specifically, adaptive silver films (ASFs) allow nanoscale restructuring of metal particles during protein deposition which yields a three-fold benefit of soft protein adsorption, protein-metal complex stabilization, and increased SERS signal. In this work ASF fabrication and characterization methods are introduced, with special attention paid to characterization methods that provide insight into the adaptive nature of the substrates, such as UV-vis spectrophotometry, field-emission scanning electron microscopy, atomic force microscopy, and x-ray photoelectron spectroscopy. These ASF substrates show SERS enhancement factors in the range of 10⁶ for an area-averaged signal, and have been successfully used for sub-monolayer protein detection. The addition of a thick metal layer in the ASF fabrication structure typically increases the SERS signal by a factor of four or five. Finally, several examples of current SERS protein studies using ASF substrates are provided.

Citation Download Citation

Mark D. Thoreson, Vladimir P. Drachev, Eldar N. Khaliullin, Vishal Nashine, Dor Ben-Amotz, V. Jo Davisson, and Vladimir M. Shalaev "Adaptive SERS substrates for protein sensing", Proc. SPIE 5593, Nanosensing: Materials and Devices, (29 December 2004); https://doi.org/10.1117/12.579779

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KEYWORDS

Proteins

Metals

Surface enhanced Raman spectroscopy

Silver

Particles

Nanostructures

Scanning electron microscopy

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