Signaling of metabolic changes in algae by means of light emission variations

Anna Jaskowska; Zbigniew Gorski; Andrzej Dudziak

doi:10.1117/12.577199

26 August 2004 Signaling of metabolic changes in algae by means of light emission variations

Anna Jaskowska, Zbigniew Gorski, Andrzej Dudziak

Proceedings Volume 5566, Optical Security and Safety; (2004) https://doi.org/10.1117/12.577199
Event: Optical Security and Safety, 2003, Warsaw, Poland

Abstract

This work deals with light emission changes from Characeae cells induced in them by mechanical injury, metabolites e.g. ascorbic acid (AsA) and local anaesthetic (LA) - lignocaine. Two optoelectronic methods: (1) the single photon counting (SPC) with an EMI Thorn 9558A (340 - 800 nm) photomultiplier, and (2) the single photon imaging (SPCI) using Molecular Light Imager "Night Owl" LB 981 EG&G Berthold were employed for ultraweak light emission changes record. These methods were used as the complementary ones. Spontaneous ultraweak emission from plants (kept in darkness) as a photonic response to the perturbation of their oxidative metabolism was studied. The reagents used here are antioxidants quenching singlet oxygen ¹O₂*, and lignocaine is an efficient scavenger of OH*. An increase of the emission intensity was observed both in the case of the mechanical injury and action of AsA or LA. It is pointed out that the use of the SPC is justifiable for samples requiring a high time resolution while the use of the SPCI for samples requiring determination of the space-distribution of emission. The procedure of analysis of temporal-spatial changes in the light emission by objects with a complex shape has been elaborated. It is suggested that ultraweak light emission changes could be considered as an optical determinant of homeostasis security state in plant cells.

Citation Download Citation

Anna Jaskowska, Zbigniew Gorski, and Andrzej Dudziak "Signaling of metabolic changes in algae by means of light emission variations", Proc. SPIE 5566, Optical Security and Safety, (26 August 2004); https://doi.org/10.1117/12.577199

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