Condensation of hydrodynamically stretched DNA using single-molecule fluorescence imaging and optical tweezers

Eirini Theofanidou; Tsueu Ju Su; Jochen Arlt; David T. F. Dryden; William J. Hossack; Jason Crain; Wilson C. K. Poon

doi:10.1117/12.558896

18 October 2004 Condensation of hydrodynamically stretched DNA using single-molecule fluorescence imaging and optical tweezers

Eirini Theofanidou, Tsueu Ju Su, Jochen Arlt, David T. F. Dryden, William J. Hossack, Jason Crain, Wilson C. K. Poon

Author Affiliations +

Proceedings Volume 5514, Optical Trapping and Optical Micromanipulation; (2004) https://doi.org/10.1117/12.558896
Event: Optical Science and Technology, the SPIE 49th Annual Meeting, 2004, Denver, Colorado, United States

Abstract

We have observed a latency time of the order 10 s in the spermidine-induced condensation of YOYO-1-labelled DNA in flow. Higher flow speeds, longer DNA and higher salt all led to an increase in the latency time. We propose that two effects may be relevant to these observations (1) the competition between spermidine and YOYO-1 for DNA binding sites, and (2) the bending of DNA upon spermidine binding, so that flow-induced straightening of the DNA weakens spermidine's affinity for DNA. Moreover, literature data on the mechanical properties of condensed DNA chains suggest that the hydrodynamic drag forces in our experiments should indeed significantly perturb condensation kinetics.

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Eirini Theofanidou, Tsueu Ju Su, Jochen Arlt, David T. F. Dryden, William J. Hossack, Jason Crain, and Wilson C. K. Poon "Condensation of hydrodynamically stretched DNA using single-molecule fluorescence imaging and optical tweezers", Proc. SPIE 5514, Optical Trapping and Optical Micromanipulation, (18 October 2004); https://doi.org/10.1117/12.558896

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