Demonstration of optical microfluidics

Kenneth T. Kotz; Konstantinos S. Kalogerakis; Kyle A. Noble; Sarah E. Smith; Gregory W. Faris

doi:10.1117/12.538890

22 June 2004 Demonstration of optical microfluidics

Kenneth T. Kotz, Konstantinos S. Kalogerakis, Kyle A. Noble, Sarah E. Smith, Gregory W. Faris

Proceedings Volume 5328, Microarrays and Combinatorial Techniques: Design, Fabrication, and Analysis II; (2004) https://doi.org/10.1117/12.538890
Event: Biomedical Optics 2004, 2004, San Jose, CA, United States

Abstract

We demonstrate a novel method for the control of small droplets using laser-based heating. Temperature dependent interfacial surface tensions were the primary force used to move droplets. With this approach, ~1.7 μL to 14 pL droplets were moved on a bare, unmodified polystyrene surface, at speeds of up to 3 mm/s. Upon contact, droplets spontaneously fused and rapidly mixed within 33 ms. We performed an optical absorption-based protein assay using horseradish peroxidase and a chromogenic substrate (ABTS), and readily detected as little as ~125 attomoles of reacting enzyme.

Citation Download Citation

Kenneth T. Kotz, Konstantinos S. Kalogerakis, Kyle A. Noble, Sarah E. Smith, and Gregory W. Faris "Demonstration of optical microfluidics", Proc. SPIE 5328, Microarrays and Combinatorial Techniques: Design, Fabrication, and Analysis II, (22 June 2004); https://doi.org/10.1117/12.538890

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