Nonlinear interferometric vibrational imaging of molecular species

Jeremy S. Bredfeldt; Daniel L. Marks; Claudio Vinegoni; Selezion Hambir; Dana Dlott; Stephen Boppart

doi:10.1117/12.527834

1 July 2004 Nonlinear interferometric vibrational imaging of molecular species

Jeremy S. Bredfeldt, Daniel L. Marks, Claudio Vinegoni, Selezion Hambir, Dana Dlott, Stephen Boppart

Proceedings Volume 5321, Biomedical Vibrational Spectroscopy and Biohazard Detection Technologies; (2004) https://doi.org/10.1117/12.527834
Event: Biomedical Optics 2004, 2004, San Jose, CA, United States

Abstract

Vibrationally-sensitive spectroscopic techniques are becoming important clinical tools for real-time, in vivo diagnostics. The molecular information made available with these techniques can provide early diagnostic signs of disease, often before morphological changes occur. We model and experimentally demonstrate a new technique for measuring optical spectroscopy signals using interferometric ranging. This new technique, nonlinear interferometric vibrational imaging (NIVI), uses principles from coherent anti-Stokes Raman scattering (CARS) spectroscopy and optical coherence tomography (OCT) to achieve cross-sectional imaging of the distribution of specific molecular species within a sample. Two CARS signals are generated, one from a known reference molecular species and a second from the unknown molecules in a sample. These coherent signals are interfered with each other using an interferometer setup. The intensity envelope of the interference signal provides a measure of the concentration of selected bonds present in the sample focal volume. The interference fringes themselves can provide phase information that will allow for the exact reconstruction of the vibrational characteristics of the molecules in the sample focal volume. Theoretical background to CARS interferometry is presented, the experimental laser systems are described, and a depth-resolved scan line of a benzene filled cuvette is demonstrated. The experimental results show close resemblance to the theoretical models. The advantages of NIVI over existing vibrational imaging systems and its clinical implications are discussed.

Citation Download Citation

Jeremy S. Bredfeldt, Daniel L. Marks, Claudio Vinegoni, Selezion Hambir, Dana Dlott, and Stephen Boppart "Nonlinear interferometric vibrational imaging of molecular species", Proc. SPIE 5321, Biomedical Vibrational Spectroscopy and Biohazard Detection Technologies, (1 July 2004); https://doi.org/10.1117/12.527834

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KEYWORDS

Raman spectroscopy

Molecules

Interferometry

Optical coherence tomography

Polarization

Spectroscopy

Confocal microscopy

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