We detected the serum of patient's and normal using auto-fluorescence and Raman spectroscopy. The serum spectrum was excited by laser of the wavelength 488.0 nm and 514.5 nm. Compared with the normal serum, we can observe the apparent differences of autofluorescence-Raman spectroscopy: the majority of the fluorescence spectrum did not have violent alteration, but three Raman peak had disappeared or very weak. After operation, the Raman spectrum of patient's serum is similar to the normal. And fluorescence peak's red shift, α-value also provide the reference for future research. We assume that following the progression of the tumor, β-value will decrease following the aggravation of cancer. The result of spectrum analysis is accordance with the clinical diagnosis.
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