Analysis of normal and diseased liver tissue using auto-fluorescence and Raman spectroscopy

Xiaozhou Li; Chunde Jia; Junxiu Lin; Youping Kang

doi:10.1117/12.546248

4 December 2003 Analysis of normal and diseased liver tissue using auto-fluorescence and Raman spectroscopy

Xiaozhou Li, Chunde Jia, Junxiu Lin, Youping Kang

Proceedings Volume 5255, Biomolecular Photonics and Multidimensional Microscopy; (2003) https://doi.org/10.1117/12.546248
Event: Biomolecular Photonics and Multidimensional Microscopy, 2003, Wuhan, China

Abstract

In this paper, laser induced human serum Raman spectra of liver cancer are measured. The spectra differences in serum from normal people and liver cancer patients are analyzed. For the typical spectrum of normal serum, there are three sharp Raman peaks and relative intensity of Raman peaks excited by 514.5 nm is higher than that excited by 488.0 nm. However, for the Raman spectrum of liver cancer serum there are no peaks or very weak Raman peaks at the same positions. Results from more than two hundred case measurements show that clinical diagnostic accuracy is 92.86%. And then, the liver fibrosis and liver cirrhosis are studied applying the technology of LIF. To liver cirrhosis, the shape of Raman peak is similar to normal and fluorescence spectrum is similar to that of liver cancer from statistic data. The experiment indicates that there is notable fluorescence difference between the abnormal and normal liver tissue and have blue shift in fluorescence peak. These results have important reference values to explore the method of laser spectrum diagnosis.

Citation Download Citation

Xiaozhou Li, Chunde Jia, Junxiu Lin, and Youping Kang "Analysis of normal and diseased liver tissue using auto-fluorescence and Raman spectroscopy", Proc. SPIE 5255, Biomolecular Photonics and Multidimensional Microscopy, (4 December 2003); https://doi.org/10.1117/12.546248

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