Design and application of fiber optic evanescent wave biosensor

Huijie Huang; Junhui Zhai; Yongkai Zhao; Ruifu Yang; Bingqiang Ren; Zhaogu Cheng; Longlong Du; Dunwu Lu

doi:10.1117/12.546217

8 December 2003 Design and application of fiber optic evanescent wave biosensor

Huijie Huang, Junhui Zhai, Yongkai Zhao, Ruifu Yang, Bingqiang Ren, Zhaogu Cheng, Longlong Du, Dunwu Lu

Proceedings Volume 5254, Third International Conference on Photonics and Imaging in Biology and Medicine; (2003) https://doi.org/10.1117/12.546217
Event: Third International Conference on Photonics and Imaging in Biology and Medicine, 2003, Wuhan, China

Abstract

A fiber-optic biosensor is developed based on the principle of evanescent wave while light propagates in optical fiber. The biosensor uses a red laser diode at 636.85 nm for exciting Cy5 fluorescent dye. Sensitivity limit of 0.01 nnmol/l is obtained from the detection of serial Cy5 solutions with various concentrations. In log-to-log plot, excellent linear response characteristic is seen in the Cy5 concentrations ranging from 0.01 nmlo/l to 100 nmol/l. And a good result of signal-to-noise ratio of 4.61 is obtained when the biosensor is used to measure Legionella pneumophila solution of 0.01 μmol/l. All the results are comparable with those that are obtained by a commercial biochip scanner GeneTAC 1000.

Citation Download Citation

Huijie Huang, Junhui Zhai, Yongkai Zhao, Ruifu Yang, Bingqiang Ren, Zhaogu Cheng, Longlong Du, and Dunwu Lu "Design and application of fiber optic evanescent wave biosensor", Proc. SPIE 5254, Third International Conference on Photonics and Imaging in Biology and Medicine, (8 December 2003); https://doi.org/10.1117/12.546217

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