Time-resolved multifocal multiphoton microscopy

Sandrine Leveque-Fort; J. Penon; Marie-Pierre Fontaine-Aupart; Gerard Roger; Patrick Georges

doi:10.1117/12.500627

9 October 2003 Time-resolved multifocal multiphoton microscopy

Sandrine Leveque-Fort, J. Penon, Marie-Pierre Fontaine-Aupart, Gerard Roger, Patrick Georges

Proceedings Volume 5139, Confocal, Multiphoton, and Nonlinear Microscopic Imaging; (2003) https://doi.org/10.1117/12.500627
Event: European Conferences on Biomedical Optics 2003, 2003, Munich, Germany

Abstract

Two photon microscopy is a powerful tool for cells or tissues imaging. However it presents the drawback of being a laser-scanning technique leading to long time acquisition for 3D images. To preserve biological samples from too long experiments and provide a more complete spectroscopic tool we developed a time-resolved multifocal multiphoton microscope. This setup allows us to speed up the acquisition and gives both intensity and lifetime images for all multifocal points.

Citation Download Citation

Sandrine Leveque-Fort, J. Penon, Marie-Pierre Fontaine-Aupart, Gerard Roger, and Patrick Georges "Time-resolved multifocal multiphoton microscopy", Proc. SPIE 5139, Confocal, Multiphoton, and Nonlinear Microscopic Imaging, (9 October 2003); https://doi.org/10.1117/12.500627

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