Two-dimensional thermal analysis for freezing of plant and animal cells by high-speed microscopic IR camera

Junko Morikawa; Toshimasa Hashimoto; Eita Hayakawa; Hideyuki Uemura

doi:10.1117/12.487047

1 April 2003 Two-dimensional thermal analysis for freezing of plant and animal cells by high-speed microscopic IR camera

Junko Morikawa, Toshimasa Hashimoto, Eita Hayakawa, Hideyuki Uemura

Author Affiliations +

Proceedings Volume 5073, Thermosense XXV; (2003) https://doi.org/10.1117/12.487047
Event: AeroSense 2003, 2003, Orlando, Florida, United States

Abstract

Using a high speed IR camera for temperature sensor is a powerful new tool for thermal analysis in the cell scale biomaterials. In this study, we propose a new type of two-dimensional thermal analysis by means of a high speed IR camera with a microscopic lens, and applied it to the analysis of freezing of plant and animal cells. The latent heat on the freezing of super cooled onion epidermal cell was randomly observed by a unit cell size, one by one, under a cooling rate of 80degC/min with a spatial resolution of 7.5m. The freezing front of ice formation and the thermal diffusion behavior of generated latent heat were analyzed. As a result it was possible to determine simultaneously the intercellular/intracellular temperature distribution, the growing speed of freezing front in a single cell, and the thermal diffusion in the freezing process of living tissue. A new measuring system presented here will be significant in a transient process of biomaterials. A newly developed temperature wave methods for the measurement of in-plane thermal diffusivity was also applied to the cell systems.

Citation Download Citation

Junko Morikawa, Toshimasa Hashimoto, Eita Hayakawa, and Hideyuki Uemura "Two-dimensional thermal analysis for freezing of plant and animal cells by high-speed microscopic IR camera", Proc. SPIE 5073, Thermosense XXV, (1 April 2003); https://doi.org/10.1117/12.487047

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