Applications of fluorescence spectroscopy and dynamic light scattering in biotechnology of camptothecins: promising anticancer drugs

Stefan Kruszewski; Thomas G. Burke

doi:10.1117/12.501386

1 April 2003 Applications of fluorescence spectroscopy and dynamic light scattering in biotechnology of camptothecins: promising anticancer drugs

Stefan Kruszewski, Thomas G. Burke

Author Affiliations +

Proceedings Volume 5064, Lightmetry 2002: Metrology and Testing Techniques Using Light; (2003) https://doi.org/10.1117/12.501386
Event: Lightmetry 2002, 2002, Warsaw, Poland

Abstract

Methods of optical measurements are widely used in biomedical research. Applications of these methods in determining of properties of camptothecins -- S-phase anticancer agents are described in this paper. Under physiological conditions CPT hydrolyses, and converts into inactive carboxylate form. Decrease of active form of CPT occurs especially fast in plasma of human blood. One of camptothecin analogues -- DB-67, exhibits, in contrary to CPT, relatively high stability in human blood. Fluorescence spectroscopy methods were used to determine physical and to predict biological properties of camptothecins, including DB-67. The dynamic light scattering (DLS) methods were applied to control the sizes of liposomes used as DB-67 delivery system.

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Stefan Kruszewski and Thomas G. Burke "Applications of fluorescence spectroscopy and dynamic light scattering in biotechnology of camptothecins: promising anticancer drugs", Proc. SPIE 5064, Lightmetry 2002: Metrology and Testing Techniques Using Light, (1 April 2003); https://doi.org/10.1117/12.501386

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