Hyper-Rayleigh scattering (HRS) as a mechanism for biphotonic fluorescence excitation in microscopy

Melville R. V. Sahyun; Matthew Goertz

doi:10.1117/12.472868

10 July 2003 Hyper-Rayleigh scattering (HRS) as a mechanism for biphotonic fluorescence excitation in microscopy

Melville R. V. Sahyun, Matthew Goertz

Proceedings Volume 4963, Multiphoton Microscopy in the Biomedical Sciences III; (2003) https://doi.org/10.1117/12.472868
Event: Biomedical Optics, 2003, San Jose, CA, United States

Abstract

We have observed fluorescence from Coumarin 334 in solution using light of twice an actinic wavelength for excitation. The effect is demonstrated to be biphotonic, but is observed at optical powers below those required for resonant biphotonic excitation, and with an incoherent source. We infer operation of HRS. By extrapolation we propose that this effect should contribute to fluorescence under usual conditions for multiphoton fluorescence microscopy, and that it might be exploited in fluorescence microscopy under conditions where usual mechanisms are inapplicable.

Citation Download Citation

Melville R. V. Sahyun and Matthew Goertz "Hyper-Rayleigh scattering (HRS) as a mechanism for biphotonic fluorescence excitation in microscopy", Proc. SPIE 4963, Multiphoton Microscopy in the Biomedical Sciences III, (10 July 2003); https://doi.org/10.1117/12.472868

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