Photosensitizer delivery into tissue and cells by simultaneous application of laser-induced stress wave and heating

Makoto Ogura; Mitsuhiro Terakawa; Shunichi Sato; Hiroshi Ashida; Minoru Obara

doi:10.1117/12.476137

12 September 2003 Photosensitizer delivery into tissue and cells by simultaneous application of laser-induced stress wave and heating

Makoto Ogura, Mitsuhiro Terakawa, Shunichi Sato, Hiroshi Ashida, Minoru Obara

Author Affiliations +

Proceedings Volume 4949, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems XIII; (2003) https://doi.org/10.1117/12.476137
Event: Biomedical Optics, 2003, San Jose, CA, United States

Abstract

In order to enhance the efficacy of photodynamic therapy for skin diseases, we previously demonstrated that the simultaneous application of heating and a laser-induced stress wave (LISW) accelerated skin permeabilization of porfimer sodium (Photofirn) in in-vivo experiments. In this study, to investigate the uptake of Photofrin by cells within skin, we carried out in-vitro experiments using fibroblast-like cells. Immediately after replacing culture medium with 43 degrees C phosphate buffered saline solution of Photofrin in a well, the cells were exposed to a LISW that was generated by irradiation of a nanosecond laser pulse onto a target attached on the back of the well. Uptake of Photofrin within the cells was observed with a confocal laser scanning microscope, while the cells were stained with DAPI (4',6-diamidino-2-phenylindole) to evaluate the survival rate. The largest uptake was observed for the simultaneous application (heating + LISW) with high survival rates (more than 95%). These results suggested that Photofrin was transported into fibroblasts within skin. In this method, irradiation of low-fluence laser was effective to enhance uptake, and therefore optical fiber can be used for laser delivery.

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Makoto Ogura, Mitsuhiro Terakawa, Shunichi Sato, Hiroshi Ashida, and Minoru Obara "Photosensitizer delivery into tissue and cells by simultaneous application of laser-induced stress wave and heating", Proc. SPIE 4949, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems XIII, (12 September 2003); https://doi.org/10.1117/12.476137

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KEYWORDS

Skin

Fiber lasers

Laser therapeutics

Laser tissue interaction

Photodynamic therapy

Confocal microscopy

Laser scanners

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