Preparation, characterization, and fabrication of uniform coated Y2O2S:RE3+ up-converting phosphor particles for biological detection applications

Shang Li; Hans Feindt; George Giannaras; Russell Scarpino; Sal Salamone; R. Sam Niedbala

doi:10.1117/12.451590

4 November 2002 Preparation, characterization, and fabrication of uniform coated Y₂O₂S:RE³⁺ up-converting phosphor particles for biological detection applications

Shang Li, Hans Feindt, George Giannaras, Russell Scarpino, Sal Salamone, R. Sam Niedbala

Author Affiliations +

Proceedings Volume 4809, Nanoscale Optics and Applications; (2002) https://doi.org/10.1117/12.451590
Event: International Symposium on Optical Science and Technology, 2002, Seattle, WA, United States

Abstract

The unique features of Up-Converting Phosphor Technology (UPTT) have been demonstrated in several particle-based biological assays in recent years. The core-shell structured up-converting phosphor reagents were developed as ultra sensitive labels for biological detection applications. Submicron yttrium oxysulfide (Y₂O₂S) spheres co-doped with trivalent rare earth ion pairs (RE³⁺), e.g. Yb³⁺-Er³⁺ and Yb³⁺-Tm³⁺, were prepared using homogenous precipitation followed by a series of fluidized bed processes. These particles are capable of emitting intense phosphorescence when excited by 950 or 980 nm infrared diode lasers, possessing anti-Stokes shifts greater than 150 nm. We encapsulated Y₂O₂S:RE³⁺ particles with uniform silica shells to passivate the surface and to enhance the dispersibility. The surface of silica-coated particles was further modified with functionalized silanes to provide desired chemical groups (amino, thiol, or carboxyl) for covalent coupling to biomolecules. The functional group densities of the phosphor reagents were characterized using spectroscopic techniques. Fabricated up-converting phosphor reagents can be attached to biomolecules using standard bioconjugation techniques.

Citation Download Citation

Shang Li, Hans Feindt, George Giannaras, Russell Scarpino, Sal Salamone, and R. Sam Niedbala "Preparation, characterization, and fabrication of uniform coated Y₂O₂S:RE³⁺ up-converting phosphor particles for biological detection applications", Proc. SPIE 4809, Nanoscale Optics and Applications, (4 November 2002); https://doi.org/10.1117/12.451590

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available