Changes in quantitative oxygen uptake rate and redox state of cytochromes as a function of normal, critical, and anoxic conditions

Klaus H. Frank; Valerie Steinbach; Marc Dammann; Manfred D. Kessler

doi:10.1117/12.469463

5 June 2002 Changes in quantitative oxygen uptake rate and redox state of cytochromes as a function of normal, critical, and anoxic conditions

Klaus H. Frank, Valerie Steinbach, Marc Dammann, Manfred D. Kessler

Author Affiliations +

Proceedings Volume 4623, Functional Monitoring and Drug-Tissue Interaction; (2002) https://doi.org/10.1117/12.469463
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

Isolated mitochondria were used to investigate quantitative oxygen uptake rate during oxidative phosphorylation. At the PO₂ of 70 mmHg the reaction was initiated. During the initial part of oxidative phosphorylation the turnover rate increased. The recorded PO₂ slope decayed linearly over time from 60 to about 10 mmHg, which is correlated to a constant oxygen uptake rate. Below 10 mmHg the PO₂ deviated from linearity indicating a decreasing O₂ uptake of mitochondria. No change in redox state of respiratory enzymes was observed. At very low PO₂ values the scattering of mitochondria decreased and finally at total anoxia a very fast drop of backscattered light intensity and a total reduction of cytochrome was observed.

Citation Download Citation

Klaus H. Frank, Valerie Steinbach, Marc Dammann, and Manfred D. Kessler "Changes in quantitative oxygen uptake rate and redox state of cytochromes as a function of normal, critical, and anoxic conditions", Proc. SPIE 4623, Functional Monitoring and Drug-Tissue Interaction, (5 June 2002); https://doi.org/10.1117/12.469463

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