High-sensitivity detection of capillary electrophoresis with multiphoton excitation

Ling Fu; Qiang Lu; Hong Chen; Tongsheng Chen; Shaoqun Zeng; Qingming Luo

doi:10.1117/12.462561

12 April 2002 High-sensitivity detection of capillary electrophoresis with multiphoton excitation

Ling Fu, Qiang Lu, Hong Chen, Tongsheng Chen, Shaoqun Zeng, Qingming Luo

Proceedings Volume 4536, International Workshop on Photonics and Imaging in Biology and Medicine; (2002) https://doi.org/10.1117/12.462561
Event: International Workshop on Photonics and Imaging in Biology and Medicine, 2001, Wuhan, China

Abstract

Multiphoton excited fluorescence detection is a powerful tool for probing chemistry and structure deep within biological tissue, for performing sensitive measurement on deep-UV (ex 200-300 nm) specimen. We present an approach for rapid analysis of Rhodamine B using multiphoton excited fluorescence detection coupled to capillary electrophoresis separations. In this highly versatile approach, Rhodamine B is excited through the nearly simultaneous absorption of two low-energy photons, fluorescence can be detected efficiently with low background. In these studies, Rhodamine B is fractionated in several minutes, with mass detection limits as low as 10 amol (2nM). This approach is demonstrated to be a powerful tool for analyzing the complex biological samples of minute quantities.

Citation Download Citation

Ling Fu, Qiang Lu, Hong Chen, Tongsheng Chen, Shaoqun Zeng, and Qingming Luo "High-sensitivity detection of capillary electrophoresis with multiphoton excitation", Proc. SPIE 4536, International Workshop on Photonics and Imaging in Biology and Medicine, (12 April 2002); https://doi.org/10.1117/12.462561

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