Fourier interferometry with spectral fluorescence imaging for the study of mitochondrial distribution and function in living normal/malignant cells

Joseph G. Hirschberg; Elli Kohen; Cahide Kohen; Ceren Oernek; John P. Berry

doi:10.1117/12.446674

24 October 2001 Fourier interferometry with spectral fluorescence imaging for the study of mitochondrial distribution and function in living normal/malignant cells

Joseph G. Hirschberg, Elli Kohen, Cahide Kohen, Ceren Oernek, John P. Berry

Author Affiliations +

Proceedings Volume 4434, Hybrid and Novel Imaging and New Optical Instrumentation for Biomedical Applications; (2001) https://doi.org/10.1117/12.446674
Event: European Conferences on Biomedical Optics, 2001, Munich, Germany

Abstract

The advantages of measuring both the excitation spectrum and emission spectrum of living cells' fluorescence are reviewed together with interferometric methods. Mitochondria in yeast cells are described as examples of Biomedical methods, also including mammalian cells. Mitochondria in human hepatic cells, murine mast cells and human keratinocytes are shown using the vital stains DASPMI, Mitotracker Green and TMRE. An example in Biotechnology is the study of photosynthetic algae, used in the production of hydrogen.

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Joseph G. Hirschberg, Elli Kohen, Cahide Kohen, Ceren Oernek, and John P. Berry "Fourier interferometry with spectral fluorescence imaging for the study of mitochondrial distribution and function in living normal/malignant cells", Proc. SPIE 4434, Hybrid and Novel Imaging and New Optical Instrumentation for Biomedical Applications, (24 October 2001); https://doi.org/10.1117/12.446674

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