Separation of myoglobin and hemoglobin in isolated perfused pig heart

Robert Rauh M.D.; Michael Hiller; Tobias Trinks; Manfred D. Kessler

doi:10.1117/12.447147

31 October 2001 Separation of myoglobin and hemoglobin in isolated perfused pig heart

Robert Rauh M.D., Michael Hiller, Tobias Trinks, Manfred D. Kessler

Proceedings Volume 4432, Diagnostic Optical Spectroscopy in Biomedicine; (2001) https://doi.org/10.1117/12.447147
Event: European Conferences on Biomedical Optics, 2001, Munich, Germany

Abstract

For the quantitative determination of hemoglobin concentration in heart muscle it is important to distinguish between myoglobin and hemoglobin, two dyes with very similar optical absorption properties. With an isolated perfused pig heart model and EMPHO II SSK we measured tissue spectra in the visible range before and after adding erythrocytes to the perfusate. By calculating light intensity differences we were able to show spatial hemoglobin distribution in heart muscle.

Citation Download Citation

Robert Rauh M.D., Michael Hiller, Tobias Trinks, and Manfred D. Kessler "Separation of myoglobin and hemoglobin in isolated perfused pig heart", Proc. SPIE 4432, Diagnostic Optical Spectroscopy in Biomedicine, (31 October 2001); https://doi.org/10.1117/12.447147

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