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A novel photobleaching model was proposed, in which a D-P photobleaching mechanism was developed. By combining with the conventional D-D and D-O photobleaching mechanisms, the dependence of photobleaching rate on the excitation power is well illustrated. The validity of this model was demonstrated with GFP photobleaching experiments in cases of one-photon excitation (1PE) and two-photon excitation (2PE) respectively. Previously inexplicable experimental results published in literature were also illustrated with this new model.
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Tongsheng Chen, Shaoqun Zeng, Qingming Luo, Wei Zhou, Zhihong Zhang, "Novel photobleaching model in living cells," Proc. SPIE 4427, Imaging of Tissue Structure and Function, (22 August 2001); https://doi.org/10.1117/12.438419