Nanosensors for analysis of a single cell

Brian M. Cullum; Guy D. Griffin; Tuan Vo-Dinh

doi:10.1117/12.427943

4 June 2001 Nanosensors for analysis of a single cell

Brian M. Cullum, Guy D. Griffin, Tuan Vo-Dinh

Proceedings Volume 4254, Biomedical Diagnostic, Guidance, and Surgical-Assist Systems III; (2001) https://doi.org/10.1117/12.427943
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

Submicron fiber-optic biosensors have been developed and used to measure toxic chemicals within single cells. These sensors are fabricated by pulling the distal-end of an optical fiber to a diameter of less than one micron and coating them with antibodies to selectively bind the species of interest. This paper describes the use of these fibers to selectively measure the concentration of benzo[a]pyrene tetrol, a metabolite of benzo[a]pyrene, within individual cells of two different cell lines, human mammary carcinoma cells and rat liver epithelial cells. The results from these measurements have been used to evaluate the analytical figures of merit of these sensors such as detection limits, which were found to be 0.64 +/- 0.17 X 10^-11 M for BPT. In addition, measurements were performed both extracellularly and intracellularly, and an increase in variation of approximately 4% was determined for the intracellular measurements, relative to the extracellular measurements.

Citation Download Citation

Brian M. Cullum, Guy D. Griffin, and Tuan Vo-Dinh "Nanosensors for analysis of a single cell", Proc. SPIE 4254, Biomedical Diagnostic, Guidance, and Surgical-Assist Systems III, (4 June 2001); https://doi.org/10.1117/12.427943

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