Spatial imaging of cooperative systems in capillaries (Hb, HbO2) and cells (Redox states and changes of subcellular functional structures) in perfused liver

Markus Boehnert; Robert Rauh M.D.; Manfred D. Kessler

doi:10.1117/12.431569

4 May 2001 Spatial imaging of cooperative systems in capillaries (Hb, HbO2) and cells (Redox states and changes of subcellular functional structures) in perfused liver

Markus Boehnert, Robert Rauh M.D., Manfred D. Kessler

Author Affiliations +

Proceedings Volume 4241, Saratov Fall Meeting 2000: Optical Technologies in Biophysics and Medicine II; (2001) https://doi.org/10.1117/12.431569
Event: Saratov Fall Meeting 2000, 2001, Saratov, Russian Federation

Abstract

Light scattering in living tissues is mainly caused by subcellular particles like mitochondria. The size of mitochondria changes according to differences in the functional status. Therefore light scattering should be a useful technique for monitoring the functional state in tissues. We investigated functional parameters in our model of the isolated perfused rat liver. For the measurements of light scattering we used the EMPHO SSK Oxyscan. Backscattered light from tissue is shown in 3D images. We found an interesting relation between structures of the liver and the patterns of the relating 3D images. In addition, our underlying spectra show the redox state of cytochromes. This new method of tissue imaging should give the opportunity of new insights into liver function.

Citation Download Citation

Markus Boehnert, Robert Rauh M.D., and Manfred D. Kessler "Spatial imaging of cooperative systems in capillaries (Hb, HbO2) and cells (Redox states and changes of subcellular functional structures) in perfused liver", Proc. SPIE 4241, Saratov Fall Meeting 2000: Optical Technologies in Biophysics and Medicine II, (4 May 2001); https://doi.org/10.1117/12.431569

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