Formation of sequence-specific telomeric DNA loops via direct effects of psoralen-photosensitization on telomeres

En-Hua Cao; Ai Chen; Xueguang Sun; Xiaoyan Zhang; Jingfen Qin; Dage Liu; Chen Wang; Chunli Bai

doi:10.1117/12.403922

11 October 2000 Formation of sequence-specific telomeric DNA loops via direct effects of psoralen-photosensitization on telomeres

En-Hua Cao, Ai Chen, Xueguang Sun, Xiaoyan Zhang, Jingfen Qin, Dage Liu, Chen Wang, Chunli Bai

Author Affiliations +

Proceedings Volume 4224, Biomedical Photonics and Optoelectronic Imaging; (2000) https://doi.org/10.1117/12.403922
Event: Optics and Optoelectronic Inspection and Control: Techniques, Applications, and Instruments, 2000, Beijing, China

Abstract

DNA from HeLa cells was cleaved with Rasl and Hinf, and then purified by Bio-Gel P-2 column. Results showed that upon near-UV-irradiation, psoralen could be targeted to the repetitive sequences of telomeres. Large duplex loops with a tail were observed directly by AFM. The loop-tail junction position was assumed to be a tri- or tetra- strand DNA structure according to its apparent height, corresponding structure model was proposed. The psoralen cross-linking products increased with increase of irradiation time. The expression of p53 oncogene was significantly increased.

Citation Download Citation

En-Hua Cao, Ai Chen, Xueguang Sun, Xiaoyan Zhang, Jingfen Qin, Dage Liu, Chen Wang, and Chunli Bai "Formation of sequence-specific telomeric DNA loops via direct effects of psoralen-photosensitization on telomeres", Proc. SPIE 4224, Biomedical Photonics and Optoelectronic Imaging, (11 October 2000); https://doi.org/10.1117/12.403922

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