Cell-free protein synthesis in PDMS-glass hybrid microreactor

Takatoki Yamamoto; Teruo Fujii; Takahiko Nojima; Jong Wook Hong; Isao Endo

doi:10.1117/12.395674

18 August 2000 Cell-free protein synthesis in PDMS-glass hybrid microreactor

Takatoki Yamamoto, Teruo Fujii, Takahiko Nojima, Jong Wook Hong, Isao Endo

Proceedings Volume 4177, Microfluidic Devices and Systems III; (2000) https://doi.org/10.1117/12.395674
Event: Micromachining and Microfabrication, 2000, Santa Clara, CA, United States

Abstract

A living cell has numerous kinds of proteins while only thousands of that have been identified as of now. In order to discover and produce various proteins that are applicable to biotechnological, pharmaceutical and medical applications, cell-free protein synthesis is one of the most useful and promising techniques. In this study, we developed an inexpensive microreactor with temperature control capability for protein synthesis. The microreactor consists of a sandwich of glass-based chip and PDMS(polydimethylsiloxane) chip. The thermo control system, which is composed of a heater and a temperature sensor, is fabricated with an ITO (Indium Tin Oxide) resistive material on a glass substrate by ordinary microfabrication method based on photolithography and etching techniques. The reactor chamber and flow channels are fabricated by injection micromolding of PDMS. Since one can use thermo control system on a glass substrate repeatedly by replacing only the easily-fabricated and low-cost PDMS reactor chamber, this microreactor is quite cost effective. As a demonstration, a DNA template of a GFP (Green Fluorescent Protein) is transcribed and translated using cell-free extract prepared from Escherichia coli. As a result, GFP was successfully synthesized in the present microreactor.

Citation Download Citation

Takatoki Yamamoto, Teruo Fujii, Takahiko Nojima, Jong Wook Hong, and Isao Endo "Cell-free protein synthesis in PDMS-glass hybrid microreactor", Proc. SPIE 4177, Microfluidic Devices and Systems III, (18 August 2000); https://doi.org/10.1117/12.395674

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available