Multiphoton fluorescence spectroscopy of flourescent bioprobes and biomolecules

Ping Chin Cheng; Bai-Ling Lin; Fu-Jen Kao; Chi-Kuang Sun; Yung-Shun Wang; Tzu-Ming Liu; YiMin Wang; JianCheng Chen; Mao-Kuo Huang; Iain Johnson

doi:10.1117/12.390527

4 July 2000 Multiphoton fluorescence spectroscopy of flourescent bioprobes and biomolecules

Ping Chin Cheng, Bai-Ling Lin, Fu-Jen Kao, Chi-Kuang Sun, Yung-Shun Wang, Tzu-Ming Liu, YiMin Wang, JianCheng Chen, Mao-Kuo Huang, Iain Johnson

Author Affiliations +

Proceedings Volume 4082, Optical Sensing, Imaging, and Manipulation for Biological and Biomedical Applications; (2000) https://doi.org/10.1117/12.390527
Event: Photonics Taiwan, 2000, Taipei, Taiwan

Abstract

Multi-photon fluorescence spectra of a number of commonly used biological probes were measured in this study. Significant spectral variation has been detected between single and multi- photon excitation. The result is important for the proper selection of spectral setting/dichroic beam splitter in the set- up of a multi-photon fluorescence microscope. The information can also be useful in the detection of multi-photon fluorescence in bio-chip technology. In addition, we have investigated a few highly fluorescent bio-molecules commonly found in plant cells.

Citation Download Citation

Ping Chin Cheng, Bai-Ling Lin, Fu-Jen Kao, Chi-Kuang Sun, Yung-Shun Wang, Tzu-Ming Liu, YiMin Wang, JianCheng Chen, Mao-Kuo Huang, and Iain Johnson "Multiphoton fluorescence spectroscopy of flourescent bioprobes and biomolecules", Proc. SPIE 4082, Optical Sensing, Imaging, and Manipulation for Biological and Biomedical Applications, (4 July 2000); https://doi.org/10.1117/12.390527

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