Detection of salmonella using a real-time PCR based on molecular beacons

Wilfred Chen; Grisselle Martinez; Ashok Mulchandani

doi:10.1117/12.380515

22 March 2000 Detection of salmonella using a real-time PCR based on molecular beacons

Wilfred Chen, Grisselle Martinez, Ashok Mulchandani

Proceedings Volume 3926, Advances in Nucleic Acid and Protein Analyses, Manipulation, and Sequencing; (2000) https://doi.org/10.1117/12.380515
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

Molecular beacons are oligonucleotide probes that become fluorescent upon hybridization. We developed a new approach to detect the presence of Salmonella species using these fluorogenic reporter molecules and demonstrated their ability to discriminate between similar E. coli species in real-time PCR assays. A detection limit of 1 CFU per PCR reaction was obtained. The assays were carried out entirely in sealed PCR tubes, enabling fast and direct detection in a semiautomated format.

Citation Download Citation

Wilfred Chen, Grisselle Martinez, and Ashok Mulchandani "Detection of salmonella using a real-time PCR based on molecular beacons", Proc. SPIE 3926, Advances in Nucleic Acid and Protein Analyses, Manipulation, and Sequencing, (22 March 2000); https://doi.org/10.1117/12.380515

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