Evanescent planar waveguide detection of biological warfare simulants

David M. Sipe; Kenneth P. Schoonmaker; James N. Herron; Michael J. Mostert

doi:10.1117/12.382034

11 April 2000 Evanescent planar waveguide detection of biological warfare simulants

David M. Sipe, Kenneth P. Schoonmaker, James N. Herron, Michael J. Mostert

Proceedings Volume 3913, In-Vitro Diagnostic Instrumentation; (2000) https://doi.org/10.1117/12.382034
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

An evanescent planar waveguide Mark 1.5 instrument was used to detect simulants of biological warfare agents; ovalbumin (OV), MS2 bacteriophage, BG, and Erwinia herbicola (EH). Polyclonal tracer antibodies were labeled with the fluorescent dye, Cy5. Discrete bands of polyclonal capture antibodies were immobilized to a polystyrene planar waveguide with molded integral lenses. An ST-6 CCD camera was used for detection. OV. MS2 and BG were detected in a simultaneous 3 by 3 array; with a total of nine measurements within 6 minutes. EH was analyzed in a separate array. Results were evaluate dat the US Army Joint Field Trials V, at the Dugway Proving Grounds. Over a 10 day period, 32 unknown samples were analyzed daily for each simulant. Detection limits: OV 10 ng/ml, MS2 10⁷ pfu/ml, BG 10⁵ cfu/ml. EH was detectable at 5 X 10⁵ cfu/ml. Overall false positives were 3.0 percent. Therefore, the Mark 1.5 instrument, with a parallel array of detectors, evanescent flourescent excitation, and CCD imaging provides for rapid, sensitive, and specific detection of biological warfare agent simulants.

Citation Download Citation

David M. Sipe, Kenneth P. Schoonmaker, James N. Herron, and Michael J. Mostert "Evanescent planar waveguide detection of biological warfare simulants", Proc. SPIE 3913, In-Vitro Diagnostic Instrumentation, (11 April 2000); https://doi.org/10.1117/12.382034

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