Development of an automated handheld immunoaffinity fluorometric biosensor

Terry E. Phillips; C. Brent Bargeron; Richard C. Benson; Micah A. Carlson; Allan B. Fraser; John D. Groopman; Harvey W. Ko; Paul T. Strickland; James Velky

doi:10.1117/12.382031

11 April 2000 Development of an automated handheld immunoaffinity fluorometric biosensor

Terry E. Phillips, C. Brent Bargeron, Richard C. Benson, Micah A. Carlson, Allan B. Fraser, John D. Groopman, Harvey W. Ko, Paul T. Strickland, James Velky

Author Affiliations +

Proceedings Volume 3913, In-Vitro Diagnostic Instrumentation; (2000) https://doi.org/10.1117/12.382031
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

A new immunoaffinity fluorometric biosensor has been developed for detecting and quantifying aflatoxins, a family of potent fungi-produced carcinogens that are commonly found in a variety of agriculture products. They have also been cited as a biological agent under weapons development. The handheld, self-contained biosensor is fully automatic, highly sensitive, quick, quantitative, and requires no special storage. Concentration from 0.1 parts per billion to 50 ppb can be determined in less than 2 minutes with a 1 ml sample volume. Higher concentrations can be determined by simply reducing the sample volume. The device operates on the principles of immunoaffinity for specificity and fluorescence for a quantitative assay. The analytic procedure is flexible so that other chemical and biological analytes could be detected with minor modifications to the current device.

Citation Download Citation

Terry E. Phillips, C. Brent Bargeron, Richard C. Benson, Micah A. Carlson, Allan B. Fraser, John D. Groopman, Harvey W. Ko, Paul T. Strickland, and James Velky "Development of an automated handheld immunoaffinity fluorometric biosensor", Proc. SPIE 3913, In-Vitro Diagnostic Instrumentation, (11 April 2000); https://doi.org/10.1117/12.382031

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