Multiphoton excitation and photodynamic activity of macromolecular derivatized mTHPC

Marc Schneider; Georgi Graschew; Theo A. Roelofs; Evangelos Balanos; Stefan Rakowsky; Hanns-joerg Sinn; Peter M. Schlag

doi:10.1117/12.379882

29 March 2000 Multiphoton excitation and photodynamic activity of macromolecular derivatized mTHPC

Marc Schneider, Georgi Graschew, Theo A. Roelofs, Evangelos Balanos, Stefan Rakowsky, Hanns-joerg Sinn, Peter M. Schlag

Proceedings Volume 3909, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy IX; (2000) https://doi.org/10.1117/12.379882
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

Multiphoton excitation of photosensitizers in photodynamic therapy constitutes a promising approach, because of the increasing tissue penetration for longer wavelength of illumination. In this contribution the photodynamic activity of polyethylene glycol macromolecular derivatized mTHPC upon two-photon excitation is established. To test the photo- activity of the photosensitizer, human colon carcinoma cells, HCT-116, were incubated with 2 (mu) g/ml of mTHPC- CMPEG₄ in the nutrition medium. Subsequent pulsed laser irradiation at 784 nm focused down on growing cell monolayers restricts cell vitality clearly within 24 hours after irradiation. To investigate whether an anoxic or euoxic energy transfer mechanism is involved, a uric acid assay was performed to test for the generation of singlet oxygen. Upon single-photon excitation mTHPC-CMPEG₄ in TriPEG decomposed uric acid via the generation of singlet oxygen. Using femtosecond pulsed laser irradiation no decomposition of the uric acid was found, implying an anoxic energy transfer mechanism after tow-photon excitation. However, at present, we cannot exclude local hyperthermic effects in the cells containing the photosensitizer to contribute to the photodynamic activity upon two-photon excitation.

Citation Download Citation

Marc Schneider, Georgi Graschew, Theo A. Roelofs, Evangelos Balanos, Stefan Rakowsky, Hanns-joerg Sinn, and Peter M. Schlag "Multiphoton excitation and photodynamic activity of macromolecular derivatized mTHPC", Proc. SPIE 3909, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy IX, (29 March 2000); https://doi.org/10.1117/12.379882

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