Spectroscopic and morphologic characterization of the dentin/adhesive interface

R. M. Lemor; Michael B. Kruger; David M. Wieliczka; Jim R. Swafford; Paulette Spencer

doi:10.1117/12.345386

9 April 1999 Spectroscopic and morphologic characterization of the dentin/adhesive interface

R. M. Lemor, Michael B. Kruger, David M. Wieliczka, Jim R. Swafford, Paulette Spencer

Proceedings Volume 3608, Biomedical Applications of Raman Spectroscopy; (1999) https://doi.org/10.1117/12.345386
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

The potential environmental risks associated with dental amalgams have forced many European countries to ban their use and turn to alternative materials, composite resins. The purpose of this study was to correlate morphologic characterization of the dentin/adhesive bond with chemical analyses using micro-FTIR and micro-Raman spectroscopy. A commercial dental adhesive was placed on dentin substrates cut from extracted, unerupted human third molars. Sections of the dentin/adhesive interface were investigated using IR radiation produced at the Aladdin synchrotron source; visible radiation from Kr⁺ laser was used for the micro-Raman spectroscopy and through the use of differentially staining in conjunction with light microscopy. Due to its limited spatial resolution and the unknown samples thickness the IR results cannot be used quantitatively in determining the extent of diffusion. The result from the micro-Raman spectroscopy and light microscopy indicate exposed protein at the dentin/adhesive interface. Using a laser that reduces background fluorescence, the micro-Raman spectroscopy provides quantitative chemical and morphologic information on the dentin/adhesive interface. The staining procedure is sensitive to sites of pure protein and complements the Raman results.

Citation Download Citation

R. M. Lemor, Michael B. Kruger, David M. Wieliczka, Jim R. Swafford, and Paulette Spencer "Spectroscopic and morphologic characterization of the dentin/adhesive interface", Proc. SPIE 3608, Biomedical Applications of Raman Spectroscopy, (9 April 1999); https://doi.org/10.1117/12.345386

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KEYWORDS

Interfaces

Adhesives

Raman spectroscopy

Proteins

Infrared spectroscopy

Minerals

Infrared radiation

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