Raman microscopy and imaging of inorganic and biological materials with liquid crystal tunable filters

Pina Colarusso; Andrew Whitley; Ira W. Levin; E. Neil Lewis

doi:10.1117/12.345395

9 April 1999 Raman microscopy and imaging of inorganic and biological materials with liquid crystal tunable filters

Pina Colarusso, Andrew Whitley, Ira W. Levin, E. Neil Lewis

Proceedings Volume 3608, Biomedical Applications of Raman Spectroscopy; (1999) https://doi.org/10.1117/12.345395
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

The utility of Raman microscopy and imaging for the characterization of a variety of chemical and biological systems is discussed. Measurements have been carried out with an optical microscope coupled to a Raman spectrometer that contains light paths for both single point and imaging measurements. Laser irradiation and signal collection are implemented using epi-illumination through a single microscope objective. For point Raman microspectroscopy. In our arrangement, the laser is defocused to provide wide- field illumination. The Raman signal from within the irradiated sample area is directed through a narrow-band liquid crystal tunable filter (LCTF) and imaged onto the CCD. Spectroscopic information is obtained by recording Raman images through the LCTF over successively tuned frequencies. Raman spectra for various point within the sample thus are obtained in parallel by each pixel in the detector array. Microspectra were recorded within various sample, including bacteria. Spectroscopic features of interest were then investigated in greater spatial detail using the LCTF imaging methodology.

Citation Download Citation

Pina Colarusso, Andrew Whitley, Ira W. Levin, and E. Neil Lewis "Raman microscopy and imaging of inorganic and biological materials with liquid crystal tunable filters", Proc. SPIE 3608, Biomedical Applications of Raman Spectroscopy, (9 April 1999); https://doi.org/10.1117/12.345395

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