Raman and SERS microspectroscopy on living cells: a promising tool toward cellular drug response and medical diagnosis

Abdelilah Beljebbar; Ganesh D. Sockalingum; Hamid Morjani; Michel Manfait

doi:10.1117/12.345399

9 April 1999 Raman and SERS microspectroscopy on living cells: a promising tool toward cellular drug response and medical diagnosis

Abdelilah Beljebbar, Ganesh D. Sockalingum, Hamid Morjani, Michel Manfait

Author Affiliations +

Proceedings Volume 3608, Biomedical Applications of Raman Spectroscopy; (1999) https://doi.org/10.1117/12.345399
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

Raman spectroscopy has been sued to differentiate between sensitive and MDR-resistant cells using Raman spectral imaging with a 632.8 nm excitation wavelength. The comparison between two spectral images allowed to quantify the differences between sensitive and resistant cell lines in term of proteins, lipids when MDR phenotype is expressed. SER spectroscopy has become a powerful and non-invasive probe for investigating the molecular and cellular interaction of drugs with their targets. The comparison between these models allow to elucidate the biological effect of the drugs. The development of new types of SERS- active substrates has extended the applicability of this technique to medical diagnosis. Two kinds of SERS active substrates, characterized as 'bio-compatible' systems, can be used for investigation on single living cells: colloid suspensions and microelectrodes and island films. This methodology is used for the study of cell membrane components in interaction with the SERS substrates with the aim to understand the resistance mechanism. The constitution of a data bank will allow the follow-up of cancer and future monitoring of therapeutic intervention.

Citation Download Citation

Abdelilah Beljebbar, Ganesh D. Sockalingum, Hamid Morjani, and Michel Manfait "Raman and SERS microspectroscopy on living cells: a promising tool toward cellular drug response and medical diagnosis", Proc. SPIE 3608, Biomedical Applications of Raman Spectroscopy, (9 April 1999); https://doi.org/10.1117/12.345399

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