Scanning apertureless fluorescence microscope

Guillaume A. Lessard; T. Jason Yang; Pierre Barritault; Stephen R. Quake

doi:10.1117/12.350630

17 June 1999 Scanning apertureless fluorescence microscope

Guillaume A. Lessard, T. Jason Yang, Pierre Barritault, Stephen R. Quake

Proceedings Volume 3607, Scanning and Force Microscopies for Biomedical Applications; (1999) https://doi.org/10.1117/12.350630
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

We describe a near-field apertureless fluorescence microscope, capable of imaging fluorescent latex beads with subwavelength precision. The instrument is based on a home- built tapping-mode atomic-force microscope, to which an inverted optical microscope was added. The fact that the wavelength of the fluorescence that we observe is different from the wavelength of the illumination allows for a relatively straightforward detection mechanism. Sample images are presented, along with evidence that the observe effect is of optical origin.

Citation Download Citation

Guillaume A. Lessard, T. Jason Yang, Pierre Barritault, and Stephen R. Quake "Scanning apertureless fluorescence microscope", Proc. SPIE 3607, Scanning and Force Microscopies for Biomedical Applications, (17 June 1999); https://doi.org/10.1117/12.350630

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