Molecular DNA switches and DNA chips

Chandran R. Sabanayagam; Cristin Berkey; Uri Lavi; Charles R. Cantor; Cassandra L. Smith

doi:10.1117/12.350049

3 June 1999 Molecular DNA switches and DNA chips

Chandran R. Sabanayagam, Cristin Berkey, Uri Lavi, Charles R. Cantor, Cassandra L. Smith

Proceedings Volume 3606, Micro- and Nanofabricated Structures and Devices for Biomedical Environmental Applications II; (1999) https://doi.org/10.1117/12.350049
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

We present an assay to detect single-nucleotide polymorphisms on a chip using molecular DNA switches and isothermal rolling- circle amplification. The basic principle behind the switch is an allele-specific oligonucleotide circularization, mediated by DNA ligase. A DNA switch is closed when perfect hybridization between the probe oligonucleotide and target DNA allows ligase to covalently circularize the probe. Mismatches around the ligation site prevent probe circularization, resulting in an open switch. DNA polymerase is then used to preferentially amplify the closed switches, via rolling-circle amplification. The stringency of the molecular switches yields 10² - 10³ fold discrimination between matched and mismatched sequences.

Citation Download Citation

Chandran R. Sabanayagam, Cristin Berkey, Uri Lavi, Charles R. Cantor, and Cassandra L. Smith "Molecular DNA switches and DNA chips", Proc. SPIE 3606, Micro- and Nanofabricated Structures and Devices for Biomedical Environmental Applications II, (3 June 1999); https://doi.org/10.1117/12.350049

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