Master oscillator power amplifier diode laser and microarterial anastomosis with chromophore

Francois-Marie Lhote; Roland G. Demaria; Simone Rouy; Michel Dauzat; Marie-Claire Lauraire; Romain Roduit; Michel Prudhomme M.D.; Guy P. Delacretaz; Guilhem Godlewski M.D.

doi:10.1117/12.335784

4 January 1999 Master oscillator power amplifier diode laser and microarterial anastomosis with chromophore

Francois-Marie Lhote, Roland G. Demaria, Simone Rouy, Michel Dauzat, Marie-Claire Lauraire, Romain Roduit, Michel Prudhomme M.D., Guy P. Delacretaz, Guilhem Godlewski M.D.

Author Affiliations +

Proceedings Volume 3565, Thermal Therapy, Laser Welding, and Tissue Interaction; (1999) https://doi.org/10.1117/12.335784
Event: BiOS Europe '98, 1998, Stockholm, Sweden

Abstract

Feasibility of laser assisted micro-vascular anastomosis (LAMA) has already been proved for more than 15 years. But to our knowledge, equivalence between LAMA and conventional manual anastomosis (CMA) has never been demonstrated. With a Master Oscillator Power Amplifier diode laser (988 nm) and Fast Green FCF as chromophore, LAMA versus CMA were performed on the carotids of 92 Wistar rats. LAMA edge coaptation was achieved with three 10/0 stitches, irradiation with 3 shots of 500 mW in power and 800 nm in diameter. CMA was performed on the other side with six 10/0 stitches. At 2 months, 3 aneurysms were present on LAMA side and 3 on CMA side, the success rate was 96.7% on both sides. We established the equivalence of LAMA and CMA, in an interval of 5%, using a method of equivalence based on odds ratios and Chi-square test. Paired t-test comparing carotid flow, maximal Doppler frequencies, pulsatility and resistance index could not highlight any difference between the 2 methods. Histological analysis showed an earlier and better re-endothelialization on LAMA side. For the first time to our knowledge equivalent between LAMA and CMA has been demonstrated.

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Francois-Marie Lhote, Roland G. Demaria, Simone Rouy, Michel Dauzat, Marie-Claire Lauraire, Romain Roduit, Michel Prudhomme M.D., Guy P. Delacretaz, and Guilhem Godlewski M.D. "Master oscillator power amplifier diode laser and microarterial anastomosis with chromophore", Proc. SPIE 3565, Thermal Therapy, Laser Welding, and Tissue Interaction, (4 January 1999); https://doi.org/10.1117/12.335784

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KEYWORDS

Semiconductor lasers

Chromophores

Doppler effect

Resistance

Arteries

Oscillators

Amplifiers

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