PDT-induced apoptosis in bladder carcinoma cells

Ruediger Bachor; Ella Dumbravia Reich; Klaus Kleinschmidt; Denes Repassy; Richard E. Hautmann

doi:10.1117/12.339132

3 February 1999 PDT-induced apoptosis in bladder carcinoma cells

Ruediger Bachor, Ella Dumbravia Reich, Klaus Kleinschmidt, Denes Repassy, Richard E. Hautmann

Proceedings Volume 3563, Photochemotherapy of Cancer and Other Diseases; (1999) https://doi.org/10.1117/12.339132
Event: BiOS Europe '98, 1998, Stockholm, Sweden

Abstract

Photodynamic therapy (PDT) is a highly efficient inducer of apoptosis in EY-28 bladder carcinoma cells, resulting in extensive DNA fragmentation. Bladder carcinoma cells EY-28 (Tumorbank Heidelberg, Germany) were incubated for 1 h with 1 (mu) g AamTPPn/ml or 2 (mu) g AamTPPn/ml. After incubation cells were refed with complete medium and irradiated with 0.75 J/cm². To identify apoptotic cells, a in situ cell death detection kit POD (Boehringer Mannheim, Germany) was used. The chromatin condensation characteristic to apoptotic cells was detected by transmission electron microscopy. Using 1 (mu) g AamTPPn/ml and 2 (mu) g AamTPPn/ml (9-Acetamido-2,7,12,17- tetra-n-Porpylporphycene), respectively, and irradiation at 0.75 J/cm², a percentage of 36.9% and 54.7%, respectively, of apoptotic cells was detected.

Citation Download Citation

Ruediger Bachor, Ella Dumbravia Reich, Klaus Kleinschmidt, Denes Repassy, and Richard E. Hautmann "PDT-induced apoptosis in bladder carcinoma cells", Proc. SPIE 3563, Photochemotherapy of Cancer and Other Diseases, (3 February 1999); https://doi.org/10.1117/12.339132

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