Raman spectroscopic study of leptospiral glycolipoprotein

PeiDi Bao; Lang Bao; TianQuan Huang; XinMing Liu

doi:10.1117/12.306093

24 April 1998 Raman spectroscopic study of leptospiral glycolipoprotein

PeiDi Bao, Lang Bao, TianQuan Huang, XinMing Liu

Proceedings Volume 3257, Infrared Spectroscopy: New Tool in Medicine; (1998) https://doi.org/10.1117/12.306093
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

The Raman scattering spectra of two different samples of Leptospiral Glycoipoprotein (GLP-1 and GLP-2) which have different toxic effects have been obtained and investigated. Leptospirosis is one of the most harmful zoonosis. It is a serious public health issue in some area of Sichusan province. The two samples offer different structural informations of GLP molecules, it would be important to find the difference in contents, structures and the amino acid side - chains environment of the molecules between the two samples of GLP for understanding the different toxic effects. The intense Am I at 1651 cm^-1 and weak Am III at 1283 cm^-1 show that GLP-1 has a predominantly (alpha) -helix secondary structure. The intense Am I at 1674 cm^-1 and intense Am III at 1246 cm^-1 show that the conformation of GLP-2 has a high content of (Beta) - sheet and a low content of random - coil secondary structure. Strong Raman scattering occurs in the range 920- 980 cm^-1, belong to the C-COO vibration and the stretching of the peptide backbone. The molecules of GLP-1 has trans-gauche-trans configuration of the C-S-S-C-C linkage and the molecules of GLP-2 has trans-gauche-gauche configuration of the C-C-S-S-C-C linkage. The intensity ratio of the two tyrosine liens at 830 cm^-1 and 850 cm^-1 is 1.1 and 1.23, indicate their tyrosine reduces environment respectively. Other side-chain environment in the two samples were discussed.

Citation Download Citation

PeiDi Bao, Lang Bao, TianQuan Huang, and XinMing Liu "Raman spectroscopic study of leptospiral glycolipoprotein", Proc. SPIE 3257, Infrared Spectroscopy: New Tool in Medicine, (24 April 1998); https://doi.org/10.1117/12.306093

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KEYWORDS

Raman spectroscopy

Americium

Molecules

Raman scattering

Spectroscopy

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Analytical research

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