Optical nanocluster plasmon sensors as transducers for bioaffinity interactions

Thomas G. M. Schalkhammer; Alfred Leitner; Franz Rembert Aussenegg; Georg D. Bauer; Fritz Pittner

doi:10.1117/12.308025

1 May 1998 Optical nanocluster plasmon sensors as transducers for bioaffinity interactions

Thomas G. M. Schalkhammer, Alfred Leitner, Franz Rembert Aussenegg, Georg D. Bauer, Fritz Pittner

Proceedings Volume 3253, Biomedical Sensing and Imaging Technologies; (1998) https://doi.org/10.1117/12.308025
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

Surface enhanced absorption of metal nano-clusters enabled us to transduce bioaffinity interactions highly amplifying the optical effect of changes in sensor surface coverage. The sensors were built depositing multiple nanoscale layers: at first silver or gold were sputtered onto oxygen plasma activated polycarbonate substrates to obtain a semitransparent metal cluster layer. Alternatively the primary metal layer was built of gold colloids covalently coupled to the activated polycarbonate. Next a chemically inert distance layer was applied e.g. by polymer-spinning. Finally a second cluster layer of e.g. gold colloids was coupled via bioaffinity interactions to the surface of the inert distance layer. The optical properties of the senor were found to be dependent on the size, shape and number of the metal-clusters as well as the distance between both metal cluster layers. For biomedical sensing the number and the spatial arrangement of biorecognitive bound metal clusters was transduced into an optical signal with high sensitivity. Since the defined spatial approach of colloids to the sensor surface alone creates the signal we could visually follow molecular binding events in real time. The first setups constructed were based on lectin-hexose or antibody-antigen interaction. The analytes were quantified via a distinct change of the spectral reflectivity of the sensor chip visible to the eye or measured by a miniaturized photometric device.

Citation Download Citation

Thomas G. M. Schalkhammer, Alfred Leitner, Franz Rembert Aussenegg, Georg D. Bauer, and Fritz Pittner "Optical nanocluster plasmon sensors as transducers for bioaffinity interactions", Proc. SPIE 3253, Biomedical Sensing and Imaging Technologies, (1 May 1998); https://doi.org/10.1117/12.308025

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